High Throughput Sequencing

Next generation sequencing technologies combined with bioinformatics analysis techniques developed during the human genome projects have now been commercialised and are available to the average research group.  Life Research through our partner Micromon now offers a High Throughput Sequencing Service based around the second-generation Illumina Genome Analyzer (GAIIx) sequencing platform.

The service offers the following key features:

• Australian Made and Owned – Operated out of and owned by Monash University, the Micromon services offers one of the few Australian based high throughput services allowing you to work directly with local expertise and avoid issues with international transactions.
• State of the Art Technology – The facility is equipped with a world class second-generation Illumina Genome Analyzer (GAIIx) sequencing platform which has been tested and configured by the Micromon team.
• Results Guarantee - We are constantly working to improve the yield and quality of the sequence produced and to that end we provide a minimum sequence guarantee for every project.

Sequencing Services

The platform has an 8 “lane” capacity with each lane holding at least one sample. Each lane typically produces many tens of millions of reads, equating to between 0.3 - 4Gb of sequence.  Exact throughput depends on various factors such as application type (RNA-seq, DNA-seq, etc.) read type (paired or single end), read length, template type, (RNA/DNA etc., G+C content, insert size etc.).  Single or paired reads can be produced for most applications (see table below)

Service types and features:

Sample Preparation: library preparation is carried out using material provided by the customer and is included in the service.

Sequencing Services: Applications include: DNA sequencing (DNA-seq), mRNA sequencing (mRNA-seq), Small RNA Sequencing and Discovery (smRNA-seq), Protein-DNA Interaction discovery (ChIP-seq). Sample multiplexing technology, allowing more than one sample to be run per sequencing lane, is available for a number of these applications.  The high-level service options are shown below.

Bioinformatics Services

Through a partnership with the Victorian Bioinformatics Consortium we are able to provide high quality, local bioinformatics services to our sequencing clients.  This close relationship allows us to provide an integrated service that combines sequencing and bioinformatics into a single complete package.

Services include: Sequence assembly, sequence annotation, SNP analysis and custom services.

 To request a sample submission pack and to organise a complementary project scoping meeting please Contact Us