IP-Star® Automated Systems
- Magnetic-bead technology for great reproducibility
- Unparalleled Productivity
- True "walk-away" automation Huge time savings
- Simple operating procedure
Diagenode is an innovative leader in life sciences tools, providing products and technologies for epigenetics, genomics,and diagnostics. Diagenode offers a truly unique product, the first automated platforms for epigenetics assays, the SX-8G IP-Star® Compact and the SX-8G IP-Star®. Diagenode has designed these automation systems to make ChIP and DNA methylation studies accessible and reproducible, and ensure consistent data in every experiment. Diagenode Automated Platforms replace the numerous manual, error-prone steps of complex epigenetic applications with a reliable, highly consistent and automated process that requires minimal operator intervention. In addition, Diagenode Automated Systems minimize sample carryover, data variability, and costly errors. The platforms offer full workflow support for epigenetics research, utilizing our complete kits and laboratory-validated protocols to rapidly deliver high-quality and consistent data.
ChIP-seq data produced using Diagenode's Auto Histone ChIP-seq kit and the IP-Star® automated system
We performed in collaboration with the Genome Center (UC Davis) automated ChIP followed by next generation sequencing (Illumina Genome Analyzer) to map the epigenomic profiles of six major histone modifications in human primary T cells.
Figure 1. Auto ChIP-seq assays were performed on the SX-8G IP-Star® using primary human CD4 and CD8 T cells isolated by negative magnetic separation of peripheral blood mononuclear cells. Each Auto-ChIP sample was performed using Diagenode's Auto Histone ChIP-seq kit reagents and contained 1 µg of input chromatin. Illumina Genome Analyzer libraries were prepared and the samples were sequenced at the DNA Technologies Core at UC Davis.
ChIP-seq experiments with antibodies directed against Transcription Factors
Figure 2. ChIP-seq results were obtained with Diagenode monoclonal antibodies directed against TBP, Pol II and polyclonal antibodies directed against H3K9/14ac and H3K4me3. For TBP, ChIP was performed with 5 µg of the Diagenode antibody on sheared chromatin from 1 million HeLaS3 cells using the "Auto Histone ChIP-seq" kit on the IP-Star® automated system. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. IP'd DNA was subsequently analysed with an Illumina Genome Analyzer. Figure shows the peak distribution in 50 kb regions surrounding GAPDH. These results clearly show a localisation of TBP at the promoter of the GAPDH gene.