IRAK Antibody - Antibodies

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IRAK Antibody 0.1mg from Prosci
Code	Prosci-1007-01	Price	AU$350.00+GST
Applications	E, WB, ICC, IP	Clonality	pAb
Reactivity	H, M, R	Availability	Order today and receive it by Fri, 24 Feb 2012
Order: Tel: 1300 543 373, Email: info@liferesearch.com, Fax: 03 8648 5841 OR			Qty:
Product Type	Antibodies - Primary Antibody		Double click on above image to view full picture
Description	Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF). Protein GI No.: 8928535 Protein Accession No.: P51617 Blocking peptide: Cat. No. 1007P - IRAK Peptide Positive Controls: Cat. No. 1201 - HeLa Cell Lysate Cat. No. 1208 - THP-1 Whole Cell Lysate Note: other product sizes available
Other Name	IRAK (CT), IL-1-Receptor Associated Kinase
Application Notes	IRAK antibody can be used for Western blot at 1:1000 dilution and for immunoprecipitation with 2 to 4 ug per sample. An 80 kDa band should be detected in the lysate from non-activated cells. It has no cross response to IRAK2.
Immunogen	Antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK.
Raised In	Rabbit
Purity	Antibody is DEAE purified
Data Sheet	Click to download Data Sheet
Storage Buffer	Antibody is supplied in PBS containing 0.02% sodium azide.
Storage Instructions	IRAK antibody can be stored at 4°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
References	Cao Z; Henzel WJ; Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996;271:1128-31. Trofimova M; Sprenkle AB; Green M; Sturgill TW; Goebl MG; Harrington MA. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J Bio Chem 1996; 271: 17609-1 Jianing Huang, Xiong Gao, Shyun Li, and Zhaodan Cao. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin-1 receptor accessory protein. Proc Natl Acad Sci USA 1997;94:12829-12832 Robinson D, Shibuya K, Mui A, Zonin F, Murphy E, Sana T, Hartley SB, Menon S, Kastelein R, Bazan F, O'Garra A. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-γ production and activates IRAK and NF-κB. Immunity 1997;7:571-581

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