Annexin V-Cy3 Apoptosis Kit  400 assays

1. Detection method- Flow cytometry (Ex = 543 nm; Em = 570 nm) and fluorescence microscopy
2. Sample type- Living cells (suspension and adherant)
3. Species reactivity- Mammalian
4. Kit size- Convenient sizes (25, 100, 400 assays)

Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy.

1. Simple one step staining procedure in 10 minutes
   
2. Fast and convenient
   
3. Cy3 shows brighter red fluorescence
   
   
   

1. Annexin V-Cy3
2. 1X Binding Buffer